Your browser version may not work well with NCBI's Web applications. More information here...
1: Clin Chim Acta. 1980 Jun 10;104(2):161-7.
Related Articles, Links
Click here to read
Modified assay for determination of hydroxyproline in a tissue hydrolyzate.

Edwards CA, O'Brien WD Jr.

A modified assay for the determination of hydroxyproline in tissue is presented. The modifications greatly reduce the time required for analysis of excised tissue as first introduced by Stegemann and Stalder [1]. These modifications include a change in the technique for tissue hydrolysis and a change in the preparation of the hydroxyproline oxidizing agent. The analysis utilizes the standard addition technique, eliminating the need for correction of matrix effects between the specimen and standard. This paper attempts to give a complete detailed description of the assay such that the procedure may be repeated without requiring additional reference material.

Publication Types:
PMID: 7389130 [PubMed - indexed for MEDLINE]