Figure 1.

PIM recruitment: Lung section stained with only secondary antibody (A) show no reaction while anti-vWF antibody stained vascular endothelium (arrow). Lung sections from control rats (C) contained occasional septal ED1-positive cells (arrow) while those from BDL rats had numerous reactive cells in the septa (arrows). Immuno-electron micrograph (E) shows gold labeling (arrows) in the cytoplasm as an indication of ED-1 staining in a PIM. En: Endthelium; PIM: pulmonary intravascualr macrophage; L: lysosome. Bar: A-B: 50 μm; C-D: 100 μm; E: 1 μm.