Figure 9.

Influence of methyl prednisolone on the nuclear DNA fragmentation of L. pneumophila-infected A549 cells. Cells were pre-treated with and without methyl prednisolone (53.4 μM) for one day, and subsequently stimulated. After a 2-day incubation, nuclear DNA fragmentation stained by the TUNEL method was analyzed by flow cytometry. The nuclear DNA fragmentation of virulent strain AA100jm-infected cells without (A) and with methyl prednisolone (B), is shown with no-pretreatment/no-stimulation cells (control) (C), and 30 μM mitomycin C-exposed cells without (D) and with methyl prednisolone (E). FS indicates the cell sizes. m-P; methyl prednisolone.