Figure 7.

Caspase activity in L. pneumophila-infected A549 cells detected by colorimetric assay. One day after stimulation, infection with the virulent strain AA100jm and the avirulent strain dotO mutant at an MOI of 400, expose to 30 μM mitomycin C and no-stimulation (control), caspases activity of each cell-group was detected by colorimetric assay. The activities of caspase 3 (A), 8 (B), 9 (C), and 1 (D) are shown. Data are mean ± SD of five or six different experiments. * P < 0.05.