Figure 6.

Analysis of the nuclear DNA fragmentation in L. pneumophila-infected A549 cells by flow cytometry. Two days after treatment, A549 cells were treated for TUNEL staining and the nuclear DNA fragmentation was analyzed by flow cytometry. A549 cells infected with the virulent AA100jm (A) and the avirulent dotO mutant (B) strains at an MOI of 100, exposed to 30 μM mitomycin C (C), or not stimulated (control) (D) are shown. The nuclear DNA fragmentation is expressed as FITC staining intensity. FS indicates the cell sizes.