Figure 1.

Determination of fibrous tissue stained with aniline blue and peripheral lung sections with 2 week exposure to room air or 40% oxygen. The area of fibrous tissue stained with aniline blue (A) was selected with the color image analyzing system (B). The stained portion and the extracted portion were matched. The selected area was measured and expressed as a percentage of the total area (C). Panels D and F show staining with aniline blue. Panels E and G show areas of fibrous tissue selected with the image analyzing system. Panels D and E are from animals on room air, F and G from those on 40% oxygen, after 2 weeks of exposure. Bar = 100 μm.