Figure 8.

Activation of the cd38 promoter in the HASM cells. Luciferase activity was measured as an index of promoter activation with the Renilla luciferase activity (pRL-TK) to normalize for transfection efficiency. The normalized luciferase activity is expressed as the fold change compared to the control. Left Panel: Activation of the full length promoter and the truncated promoter. TNF-α (T) causes activation of the 3 kb promoter as compared to control (C), which is inhibited in the presence of dexamethasone (D+T). Truncated promoter: There is no activation of the truncated promoter by TNF-α. Right Panel: TNF-α causes activation of the 3 kb promoter (3 kb), but not the constructs with mutations in NF-κB or/and AP-1 site 4 (labelled NF-AP mut, NF mut and AP mut). Representative of 3 different assays.