Figure 3.

TNF-α-induced activation and specific binding of AP-1 to the consensus and cd38 putative binding sites in HASM cells. Left Panel: Nuclear protein binding to AP-1 consensus sequence and competition for AP-1 binding with unlabeled oligonucleotide consensus (AP-1 con) and putative AP-1 sequences (labeled AP1–1 to AP1–6). Note decreased binding with AP-1 con, and AP1–4 and AP1–6 unlabeled sequences. Right Panel: Nuclear protein binding to labeled oligonucleotide AP1–4 sequence (arrow on left), which is abolished in the presence of excess unlabeled oligonucleotide AP1–4 sequence (labeled AP1–4), but not by a non-specific competitor (SP1). Gel supershift with anti-c-fos antibodies (arrow and labeled Fos). Representative of 4 different assays.