Figure 3.

Natural SP-D multimers cause greater neutralization of IAV than trimers. Neutralization of IAV was assessed as in figure 1. Phil82 IAV was pre-incubated with SP-D at the indicated concentrations prior to infection of MDCK cells. Both SP-D multimers and trimers caused neutralization of IAV (* p < 0.05 compared to control); however, the effect of multimers was significantly greater than trimers (** indicates p < 0.05 compared to control or SP-D trimers). Neutralization by trimers and multimers was also significantly different when analyzed by ANOVA. Results are mean ± SEM of 5 experiments.