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IL-13-induced proliferation of airway epithelial cells: mediation by intracellular growth factor mobilization and ADAM17

Brian W Booth1,2 email, Tracy Sandifer1,3 email, Erika L Martin1 email and Linda D Martin1 email

1Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, NC, USA

2Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA

3Department of Epidemiology, School of Public Health and Community Medicine, University of Washington, Seattle, WA, USA

author email corresponding author email

Respiratory Research 2007, 8:51doi:10.1186/1465-9921-8-51

Published: 9 July 2007

Additional files

Additional file 1:

Confocal Z-stack images of NHBE cells exposed to control media for 60 min. NHBE cells were exposed to control media for 60 min, reacted with fluorescent-tagged antibodies against TGFα (red) and ADAM17 (green), as described in Materials and methods, and then imaged by confocal microscopy. Shown is a video of the Z-stack images beginning with the basal-most section of the NHBE cells and ending with the apical-most section.

Format: ZIP Size: 2.4MB Download file

Additional file 2:

Confocal Z-stack images of NHBE cells exposed to IL-13 for 60 min. NHBE cells were exposed to IL-13 (10 ng/ml) for 60 min, reacted with fluorescent-tagged antibodies against TGFα (red) and ADAM17 (green), as described in Materials and methods, and then imaged by confocal microscopy. Shown is a video of the Z-stack images beginning with the basal-most section of the NHBE cells and ending with the apical-most section.

Format: ZIP Size: 4.3MB Download file


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