Table 1

Specificity of purified CD8+ CTL a




% specific lysis b
Stimulation
 Cell Fraction
 % CD8+ cells
 % CD4+ cells
 A/HK/68
 Vac-NP
 Vac-gp120
 B/Panama
Vac-NP
 CD8+
 97.3
 1.1
 22.4
 44.3
 1.3
 0.0

CD8-
 0.6
 75.5
 NT
 1.1
 NT
 2.7
Vac-gp120
 CD8+
 90.1
 0.0
 0.34
 0.0
 9.02
 NT

CD8-
 0.0
 74.2
 NT
 3.3
 NT
 NT

a Splenocytes from Balb/c mice primed with influenza A/Taiwan (H1N1) virus were stimulated with 2 pfu/cell of recombinant Vac-NP or vaccinia-gp120 for 5 days. CD8+ T cells were obtained by positive selection using AutoMacs instrument. The percentage of CD8+ and CD4+ T cells determined by dual color flow cytometry and % lysis in purified CD8+ and CD8- fractions are presented. b Lysis of P815 (H-2d) target cells with the indicated infection was determined in the 4-hour chromium release assay. Percent specific lysis at 40:1 E:T ratio is shown. NT = not tested.

Mbawuike et al. Respiratory Research 2007 8:44   doi:10.1186/1465-9921-8-44