Figure 1.

Graphical representation of linear regression analyses to validate the quantitative real-time PCR (qPCR) assay. Dilutions of plasmid cDNA were amplified by qPCR in duplicate reactions, and standard curves were created by plotting the cycle threshold (C_t) values versus DNA quantity (Log quantity) in ng for 18s, Muc4, Muc5ac and Muc5b.