In vitro characterization of compounds on mouse CRTH2 (mCRTH2) and mouse TP (mTP) receptors. A, Competition binding analysis. B, inhibition of mCRTH2 and mTP receptor function. PGD2- or U-46619-concentration response curves in the absence and presence of the indicated compounds in mCRTH2- or mTP-expressing cells. Inserts: Schild plots. Schild analysis show potent antagonism of mCRTH2 by TM30089 (pA2 = 9.15 ± 0.11, Schild slope = 1.45 ± 0.08) whereas it does not interfere with signaling of mTP. Ramatroban is a potent antagonist on mCRTH2 (pA2 = 8.08 ± 0.14, schild slope = 0.94 ± 0.05) and mTP (pA2 = 9.36 ± 0.10, Schild slope = 1.35 ± 0.06). Experiments show mean ± SE from 5–7 independent experiments.
Uller et al. Respiratory Research 2007 8:16 doi:10.1186/1465-9921-8-16