Mixed infection and clonal representativeness of a single sputum sample in tuberculosis patients from a penitentiary hospital in Georgia

doi:10.1186/1465-9921-7-99

Respiratory Research

Volume 7

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Shamputa IC
Jugheli L
Sadradze N
Willery E
Portaels F
Supply P
Rigouts L

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Jugheli L
Sadradze N
Willery E
Portaels F
Supply P
Rigouts L
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Mixed infection and clonal representativeness of a single sputum sample in tuberculosis patients from a penitentiary hospital in Georgia

Isdore C Shamputa¹^,2 , Levan Jugheli¹^,3 , Nikoloz Sadradze³ , Eve Willery⁴ , Françoise Portaels¹ , Philip Supply⁴^* and Leen Rigouts¹^*

¹Prince Leopold Institute of Tropical Medicine, Mycobacteriology Unit, Nationalestraat 155, B-2000 Antwerp, Belgium

²Tropical Diseases Research Centre, Microbiology Unit, P. O. Box 71769, Ndola, Zambia

³International Committee of the Red Cross, 4, Kedia Str. 380054, Tbilisi, Georgia

⁴Laboratoire des Mécanismes Moléculaires de la Pathogenèse Bactérienne, INSERM U629, Institut de Biologie/Institut Pasteur de Lille, Lille, France

author email corresponding author email* Contributed equally

Respiratory Research 2006, 7:99doi:10.1186/1465-9921-7-99


Published:	17 July 2006

Additional files

Additional File 1:

DNA fingerprinting results of pre-treatment M. tuberculosis isolates with variant patterns from each of the respective 33 patients. a = first pre-treatment sample; b = second pre-treatment sample; c = third pre-treatment isolate from each patient; d = MIRU-VNTR loci are listed according to their position (in kilobases) on the H37RV genome. Alternative designations are indicated in parentheses. For isolates with minor IS6110-RFLP variations, arrows indicate additional band (s); ND = not determined; *A third allele was detected at the respective locus. Al1, Al2 = Allele 1 and Allele 2, respectively. 2S or 3S = variant alleles in locus MIRU 04, similar to those in the H37RV and BCG genomes [20].

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