Gene expression of GITR following stimulation with Ppg was increased as compared to unstimulated cells (p = 0.07). The mRNA level of the individual gene is shown as fold difference in gene expression in Ppg (10 μg/ml) stimulated as compared to unstimulated samples and compared to the housekeeping gene GAPDH. RNA was prepared as described in Methods (n = 50). Quantitative gene expression was assessed with real-time RT-PCR. Data are shown as Box- and whiskers- plots (Median, whiskers: 5% and 95%-quantile) with outliers.
Schaub et al. Respiratory Research 2006 7:40 doi:10.1186/1465-9921-7-40