Figure 2.

Bronchial reactivity in lung slices from T-bet KO- and wild-type mice. Lung slices were exposed to increasing concentrations of ACH and the decrease in cross-sectional area was quantified using digital video-microscopy. (A) At all concentrations, the maximal contraction was found to be higher in T-bet KO- (closed circles) compared to wild-type mice (Balb/C, open circles, P < 0.001). (B) The contraction velocity was also increased in airways from T-bet KO-mice at concentrations from 10^-10 to 10^-7 M ACH (P < 0.01). At 10^-6 ACH, contraction velocity (~7 %/sec) was no longer different between the two groups (n = 20 to 25 slices from 5 to 6 mice per concentration).