Figure 10.

Spontaneous changes in [Ca²⁺]_c in ASMC in lung slices. Regions of interest were defined in ASMC and spontaneous Ca²⁺-changes recorded using two-photon microscopy. Baseline fluorescence values did not differ between ASMC in lung slices from T-bet KO-mice (black columns) and wild-type mice (white columns). (B) The percentage of ASMC displaying spontaneous Ca²⁺-changes also showed no significant differences. (C) Furthermore, the amplitude of spontaneous Ca²⁺-changes (expressed as fluorescence ratio F/F₀) was similar. (D) In contrast, the incidence per min of spontaneous Ca²⁺-changes was found to be higher in ASMC from T-bet KO-mice compared to wild-type mice (n = 66 lung slices from 6 different mice for each data point, * = P < 0.05).