Figure 5.

Phagocytosing neutrophils and macrophages in lavage fluid. Photomicrographs illustrating BALF neutrophils (A) and macrophages (B) containing DNA-positive phagosomes (indicated by white arrowheads). DNA was visualized by labelling with the fluorescent membrane permeable DNA-marker Hoechst 33342. Scale bars indicate 10 μm in A and 5 μm in B. In areas of intense inflammation and neutrophil infiltration, a majority of the macrophages (M) were abundantly packed with multiple large phagosomes (C).