CD3γ mRNA levels in healthy controls and asthmatics, correlations between IL-17A and CD3γ and between IL-17A other cytokine/chemokine mRNA levels. CD3γ, IL-8, IL-17A and IL-5 mRNA levels in induced sputum of asthmatic patients were quantified as explained in figure 1 using IL-8, IL-17A and IL-5 specific primers and VIC-(β-actin) or FAM-(IL-8, IL-17A, IL-5) labeled specific probes. Asthma severity was determined as in figure 1A. Open symbols represent patients treated with corticosteroids, closed symbols represent patients without corticosteroid-treatment. Comparisons were performed using the Kruskall-Wallis test and Dunn's post test (C). Correlations were studied by Spearman non-parametric test (A-B, D). The median is indicated with a horizontal line. * = p < 0.05, ** = p < 0.01, *** = p < 0.001, and ns = not significant.
Bullens et al. Respiratory Research 2006 7:135 doi:10.1186/1465-9921-7-135