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Cigarette smoke extract dose-dependently caused induction of proinflammatory cytokine (IL-8 and IL-6) release from primary human small airway epithelial cells |
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| Proinflammatory cytokine (pg/ml) |
Treatment |
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| Control |
CSE (0.2%) |
CSE (0.5%) |
CSE (1.0%) |
TNF-α (10 ng/ml) |
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| Interleukin-8 (IL-8) |
56.2 ± 7.1 |
126 ± 40.6*** |
171 ± 21.8*** |
418 ± 52.3*** |
591 ± 76.2*** |
| Interleukin-6 (IL-6) |
87. 3 ± 7.2 |
187 ± 43.5*** |
275 ± 31.6*** |
476 ± 54.8*** |
623 ± 51.7*** |
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Primary human small airway epithelial cells (SAEC) were treated for 24 hr with cigarette smoke extract (0.2–1.0%) prepared from 1R3F research grade cigarettes and TNF-α was used as a positive control (10 ng/ml). Cells were harvested, and supernatants were collected for the measurement of IL-8 and IL-6 levels by sandwich ELISA. CSE treatment dose-dependently induced the release of both IL-8 and IL-6 from human SAEC. Data represent mean ± SEM of 3 individual experiments. ***p < 0.001 compared to control values. CSE: cigarette smoke extract. | |||||
Kode et al. Respiratory Research 2006 7:132 doi:10.1186/1465-9921-7-132 |
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