|
Flow cytometric analysis of HLA class II expression on nasal epithelial cells. |
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| Human nasal epithelial cells |
% positive |
Mean fluorescence |
|
|
||
| Normal |
2.5 ± 0.63 |
205 ± 40 |
| Normal + IFN-γ |
10.2 ± 2.69 |
1145 ± 301 |
| Asthmatic |
2.03 ± 0.47 |
193 ± 37 |
| Asthmatic + IFN-γ |
11.76 ± 1.87 |
1013 ± 189 |
|
Nasal epithelial cells were detached from culture dishes by means of nonenzymatic cell dissociation solution and were then stained with anti-HLA-DR, P, Q, FITC-conjugated monoclonal antibody. Membrane fluorescence was analysed by flow cytometry. | ||
O'Brien et al. Respiratory Research 2006 7:115 doi:10.1186/1465-9921-7-115 |
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