Figure 3.

Purification and biological activity of SP-A from human PAP material. (A): Coomassie Blue staining. (B): Western blot comparison of endotoxin-depleted (ED) SP-A preparations (1 μg) with human PAP material (1 μg) used as a positive control. (C): Effect of purified human SP-A on normal human PBMC proliferation. Cells were stimulated with PMA (2 ng/ml) and ionomycin (100 ng/ml). Stimulation Index (cpm of stimulated cells divided by cpm of corresponding unstimulated cells). Mean ± SEM of n = 12