Table 1

Effects of N-AcS, DHS and DMS on muscarine-induced reductions in luminal airway area in FVB mice. The number of experiments (lungs/slices), mean airway diameter in μm, and the luminal airway area determined 1 min (1) and 15 min (2) after stimulation with muscarine (Mus) and 1 min (3) and 15 min (4) after repeated stimulation with muscarine or after repeated stimulation with muscarine in combination with N-AcS, DHS or DMS are shown. In all experiments, the muscarine concentration was 10-6 M. Data are given as means ± S.E.M. Matched pairs (1 vs. 3; 2 vs. 4) were evaluated by Wilcoxon's rank sum test. Differences were considered as statistically significant when p < 0.05 (n.s., not significant).
Lungs /slices
 Diameter [μm]
 Area [%] 1
 Area [%] 2
 Area [%] 3
 Area [%] 4
 p (1 vs. 3)
 p (2 vs. 4)


Mus
 Mus
 n.s.
 n.s.
4/4
 206 ± 7
 31 ± 6
 38 ± 4
 38 ± 7
 28 ± 3



Mus
 Mus/N-AcS 10-6 M
 n.s.
 n.s.
6/5
 182 ± 7
 47 ± 8
 42 ± 8
 50 ± 8
 42 ± 9



Mus
 Mus/N-AcS 10-8 M
 n.s.
 n.s.
5/5
 218 ± 5
 36 ± 1
 28 ± 5
 38 ± 6
 26 ± 6



Mus
 Mus/N-AcS 10-10 M
 n.s.
 n.s.
4/4
 212 ± 22
 21 ± 8
 16 ± 5
 26 ± 6
 30 ± 5



Mus
 Mus/DHS 10-4 M
 n.s.
 n.s.
4/6
 194 ± 9
 42 ± 5
 37 ± 7
 61 ± 8
 45 ± 6



Mus
 Mus/DHS 10-6 M
 p < 0.05
 p < 0.05
7/7
 197 ± 14
 45 ± 11
 31 ± 7
 70 ± 13
 50 ± 5



Mus
 Mus/DHS 10-8 M
 p < 0.05
 p < 0.05
7/9
 181 ± 6
 39 ± 7
 29 ± 6
 50 ± 9
 47 ± 7



Mus
 Mus/DHS 10-10 M
 p < 0.05
 p < 0.05
4/8
 198 ± 6
 34 ± 3
 24 ± 3
 42 ± 4
 40 ± 4



Mus
 Mus/DMS 10-4 M
 p < 0.05
 p < 0.05
4/7
 180 ± 7
 30 ± 7
 25 ± 6
 45 ± 10
 41 ± 7



Mus
 Mus/DMS 10-6 M
 p < 0.05
 p < 0.05
4/7
 201 ± 11
 48 ± 7
 35 ± 8
 70 ± 6
 54 ± 9



Mus
 Mus/DMS 10-8 M
 p < 0.05
 p < 0.05
4/7
 206 ± 11
 31 ± 5
 25 ± 4
 54 ± 8
 50 ± 5



Mus
 Mus/DMS 10-10 M
 p < 0.05
 p < 0.05
4/6
 196 ± 15
 43 ± 6
 23 ± 4
 71 ± 11
 52 ± 7

Pfaff et al. Respiratory Research 2005 6:48   doi:10.1186/1465-9921-6-48