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Studies utilizing cDNA microarray technology to study asthma |
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| Investigator |
Microarray type |
Species/Sample size |
Summary/Key findings |
Normalization procedure |
| Number of genes |
Type of tissue |
Replications per data point |
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|
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| Zou et al.32 |
cDNA 40.000 elements |
10 monkeys Lung tissue samples |
Microarray profile of differentially expressed genes in a monkey model of allergic asthma. |
Ratios of Cy5/Cy3 multiplied to the balance coefficient of the microarray / 3 replicates |
| Brutsche et al.33 |
cDNA 600 genes |
40 subjects Mononuclear cells |
CAGE score for atopy and asthma. |
Absolute difference of the expression of CAGE scored genes 1 replicate |
| Sayama et al.38 |
cDNA 14.000 genes |
human umbilical cord mast cells |
Transcriptional response of human mast cells stimulated via the Fc (epsilon) RI and identification of mast cells as a source of IL-11. |
Array-specific normalization coefficient was calculated by centering in log base 2 space a dataset consisting of all elements with an I/D> 3-fold / 2 replicates |
| Brutsche et al.41 |
cDNA 600 genes |
40 subjects Mononuclear cells |
Apoptosis signals in atopy and asthma measured with cDNA arrays |
G.I was normalized to the housekeeping G.I / 1 replicate |
| Syed et al. 42 |
cDNA 12.228 genes |
Human CD4+ T cells |
CCR7 down-regulation in asthma |
Median G.I of each filter normalized any differences in cDNA probe activity between filters/ 1 replicate |
| Banerjee et al.43 |
cDNA 1.176 genes |
18 mice Lung tissue samples |
Gene expression profiling in inflammatory airway disease associated with elevated adenosine |
G.I was normalized to the housekeeping G.I / 2 replicates |
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Abbreviations: CAGE: Composite atopy gene expression, CCR7: Chemokine receptor 7, G.I: Gene Intensity, I/D: Intensity/Background ratio, Th: T helper, RI: Immunoglobulin receptor | ||||
Tzouvelekis et al. Respiratory Research 2004 5:26 doi:10.1186/1465-9921-5-26 |
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