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Studies utilizing microarray technology to analyze IPF |
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| Investigator |
Microarray type |
Species/Sample size |
Summary/Key findings |
Normalization procedure |
| Number of genes |
Type of tissue |
Replications per data point |
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|
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| Zuo et al.22 |
Oligonucleotide 8.400 genes |
5 patients with IPF Lung tissue specimens |
Gene expression analysis reveals matrilysin as a key regulator of PF in mice and humans. |
Gene expression levels normalized by a scaling factor multiplied to the average of differences of probe pairs (matched-mismatched) / 2 replicates |
| Kaminski et al.23 |
Oligonucleotide 6.000 genes |
30 mice Lung tissue specimens |
Global analysis of gene expression in PF reveals distinct programs regulating lung inflammation and fibrosis. |
Mean hybridization intensities of all probe sets on each array were scaled to an arbitrary, fixed level/4 replicates |
| Katsuma et al. 24 |
cDNA 4.224 genes |
22 mice Lung tissue samples |
Molecular monitoring of bleomycin-induced pulmonary fibrosis by cDNA microarray-based gene expression profiling. |
Quantified signal intensities were converted by logarithms of base two 4 replicates |
| Chambers et al. 25 |
Oligonucleotide 6.000 genes |
Human lung fibroblasts |
Global expression profiling of fibroblast responses to transforming growth factor-beta1 reveals the induction of ID1. |
Gene expression levels normalized by a scaling factor multiplied to the average of differences of probe pairs (matched-mismatched)/ 2 replicates |
| Liu et al.26 |
cDNA 10.000 genes |
12 rats Lung tissue specimens |
FIZZ1 stimulation of myofibroblast differentiation. |
Average median ratios Cy3/Cy5 values normalized to 1.0/ 4 replicates |
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Abbreviations: ID: Inhibitor of Differentiation, IPF: Idiopathic Pulmonary Fibrosis, PF: Pulmonary Fibrosis | ||||
Tzouvelekis et al. Respiratory Research 2004 5:26 doi:10.1186/1465-9921-5-26 |
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