Figure 4.

Aspergillus antigen induced goblet cell hyperplasia in wild-type, Mcp-1^-/- and Ccr2^-/- mice. Representative PAS-stained lung sections from PBS-treated wild-type mice (A) showed minimal PAS-positive staining (similar findings from Mcp-1^-/- and Ccr2^-/- control mice are not shown). Aspergillus antigen-treated wild-type (B), Mcp-1^-/- (C) and Ccr2^-/- mice (D) showed magenta staining in epithelial cells, which represents mucus. Aspergillus antigen exposure and sample collection are described in methods. Magnification, 40× objective.