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Open Access Research

The role of PPARγ in carbon nanotube-elicited granulomatous lung inflammation

Isham Huizar1, Anagha Malur1, Janki Patel1, Matthew McPeek1, Larry Dobbs2, Christopher Wingard3, Barbara P Barna1 and Mary Jane Thomassen14*

Author Affiliations

1 Department of Internal Medicine, Division of Pulmonary and Critical Care Medicine, The Brody School of Medicine, East Carolina University, 3E-149 Brody Medical Sciences Building, Greenville, NC, 27834, USA

2 Department of Pathology, East Carolina University, Greenville, USA

3 Department of Physiology, East Carolina University, Greenville, USA

4 Department of Microbiology and Immunology, East Carolina University, Greenville, USA

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Respiratory Research 2013, 14:7  doi:10.1186/1465-9921-14-7

Published: 23 January 2013

Abstract

Background

Although granulomatous inflammation is a central feature of many disease processes, cellular mechanisms of granuloma formation and persistence are poorly understood. Carbon nanoparticles, which can be products of manufacture or the environment, have been associated with granulomatous disease. This paper utilizes a previously described carbon nanoparticle granuloma model to address the issue of whether peroxisome proliferator-activated receptor gamma (PPARγ), a nuclear transcription factor and negative regulator of inflammatory cytokines might play a role in granulomatous lung disease. PPARγ is constitutively expressed in alveolar macrophages from healthy individuals but is depressed in alveolar macrophages of patients with sarcoidosis, a prototypical granulomatous disease. Our previous study of macrophage-specific PPARγ KO mice had revealed an intrinsically inflammatory pulmonary environment with an elevated pro-inflammatory cytokines profile as compared to wild-type mice. Based on such observations we hypothesized that PPARγ expression would be repressed in alveolar macrophages from animals bearing granulomas induced by MWCNT instillation.

Methods

Wild-type C57Bl/6 and macrophage-specific PPARγ KO mice received oropharyngeal instillations of multiwall carbon nanotubes (MWCNT) (100 μg). Bronchoalveolar lavage (BAL) cells, BAL fluids, and lung tissues were obtained 60 days post-instillation for analysis of granuloma histology and pro-inflammatory cytokines (osteopontin, CCL2, and interferon gamma [IFN-γ] mRNA and protein expression.

Results

In wild-type mice, alveolar macrophage PPARγ expression and activity were significantly reduced in granuloma-bearing animals 60 days after MWCNT instillation. In macrophage-specific PPARγ KO mice, granuloma formation was more extensive than in wild-type at 60 days after MWCNT instillation. PPARγ KO mice also demonstrated elevated pro-inflammatory cytokine expression in lung tissue, laser-microdissected lung granulomas, and BAL cells/fluids, at 60 days post MWCNT exposure.

Conclusions

Overall, data indicate that PPARγ deficiency promotes inflammation and granuloma formation, suggesting that PPARγ functions as a negative regulator of chronic granulomatous inflammation.