Expressions of phosphorylated Akt and Nedd4-2 in alveolar epithelial type II cells pretreated with LY294002(10 μM), Akt inhibitor(100 nM) and SGK1 inhibitor(10 μM) for 30 minutes before insulin treatment for 2 hours were measured by western blotting. The band intensity of phosphorylated Akt and Nedd4-2 were quantitated by normalized for total Akt and β-actin respectively and expressed as fold of the control. Data are presented as mean ± S.E.M.ΔP < 0.05 vs Control group;*P < 0.05 vs Insulin group; # P < 0.05 vs Insulin + Akt inhibitor group.
Deng et al. Respiratory Research 2012 13:29 doi:10.1186/1465-9921-13-29