Down-regulation of Nogo-B in airway smooth muscle of chronic asthmatic mice. Repeated sensitization and provocation resulted in airway remodeling (B) compared to wild type littermates (A). Immunohistochemistry analysis demonstrated increased expression of SM-22 in chronic asthmatic mice (D) relative to wild type littermates(C). Arrow head indicated SM-22 expression in the smooth muscle layer of the airways. Nogo-B expression in control (E) and chronic asthmatic mice (F). Arrow indicates that Nogo-B expression was lower in the smooth muscle layer in chronic asthmatic mice than that in control mice. Original magnification: ×100 for the large pictures and ×400 for the smaller inserts located in the right bottom of the large ones. G, mRNA expression of Nogo-B in the lung of control and chronic asthmatic mouse model. H, Western blot analysis demonstrated expression of Nogo-B in the lung of controls and chronic asthmatic mice. I, Protein intensity detected by Western blot with Nogo-B antibody and GAPDH. J, Effects of PDGF on Nogo-B expression in vitro. Data are mean ± SD, n = 7, *P < 0.05 as indicated.
Xu et al. Respiratory Research 2011 12:14 doi:10.1186/1465-9921-12-14