CSE inhibits Akt activation induced by CpG-C by human pDCs. pDCs (106cells/ml) were cultured for 90 min (A) without stimulant as control, (B) stimulated with CpG-C (1 μM), (C) pretreatment for 30 with LY294002 (10 μM) and then stimulated with CpG-C (1 μM), (D) stimulated with CSE (1.5%) and (E) in combination CSE (1.5%) and CpG-C (1 μM). Following fixation and permeabalization, cells were stained with Alexa Fluor 647 phospho anti-human Akt or Alexa 647 isotype control antibodies, as specified in the "Materials and methods". A representative of three independent experiments is shown. 3,000 events were counted in the pDC gate.
Mortaz et al. Respiratory Research 2009 10:47 doi:10.1186/1465-9921-10-47